Detection Of β-lactamases Enzymes Responsible For β-lactam Resistance In Some Gram Negative Bacteria By PCR

Objective(s): β- lactamases enzymes are well studied in literature since they lead to β-lactam resistance among many pathogens, hence global concern of human and animal health issues is rising. The aim study detect these kinds some gram negative bacteria isolated locally. Material methods: 30 isolate each negatives Citrobacter spp., Serratia Enterobacter spp. were obtained from different clinical samples Baghdad Al-Najaf cities through March 2020 until January 2021. Isolates identified biochemically by Vitek2 system. Lactamases encoding genes like (blaSHV  (231bp), blaIMP-1 (500), blaVIM (382), blaAmp (1150), blaCMY (1014), bla oxa23 (501), oxa51 (353bp) detected PCR. Results: It was found that (80%, 75%, 30%, 50%, 38%, 55%, 69%) spp isolates had (blaSHV, blaIMP-1, blaVIM, blaAmp, blaCMY, oxa23, oxa51), respectively. showed having oxa51) (20%, 85%, 60%, 28%, 40%, 67%, 58%), There also (60%, 25%, 33%, 81%, 73%) harbored Conclusion: Our findings revealed most locally genes, leading increase problems patients infected with those pathogens.